Nigeria suffered a 1-0 defeat to the Czech Republic in their final warm-up match before the World Cup in Russia.
Chelsea defender Tomas Kalas scored the only goal of the game in the first half.
Nigeria also lost 2-1 to England at Wembley last weekend, while the Czechs – who failed to qualify for the World Cup – lost 4-0 to Australia in their previous friendly outing.
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Nigeria will face Argentina, Croatia and Iceland in Group D in Russia.
Their opening game is against Croatia in Kaliningrad on 16 June.
Despite the defeat, there was some encouraging news for Nigeria as Leicester midfielder Wilfred Ndidi – who had been out since April with a hamstring injury – started the game.
